Plant Transcription Factor Database
Previous version: v3.0
Nicotiana benthamiana
HSF Family
Species TF ID Description
Niben101Ctg06296g00002.1HSF family protein
Niben101Ctg15726g00001.1HSF family protein
Niben101Scf00073g04002.1HSF family protein
Niben101Scf00362g00007.1HSF family protein
Niben101Scf00557g00003.1HSF family protein
Niben101Scf00868g00005.1HSF family protein
Niben101Scf01063g03005.1HSF family protein
Niben101Scf01262g00008.1HSF family protein
Niben101Scf01305g01003.1HSF family protein
Niben101Scf01372g07005.1HSF family protein
Niben101Scf01529g04008.1HSF family protein
Niben101Scf01609g00003.1HSF family protein
Niben101Scf01639g04001.1HSF family protein
Niben101Scf01777g02001.1HSF family protein
Niben101Scf02249g05012.1HSF family protein
Niben101Scf02821g03014.1HSF family protein
Niben101Scf03038g13022.1HSF family protein
Niben101Scf03326g02004.1HSF family protein
Niben101Scf03438g02001.1HSF family protein
Niben101Scf03488g02009.1HSF family protein
Niben101Scf03518g05008.1HSF family protein
Niben101Scf03662g00006.1HSF family protein
Niben101Scf03923g03003.1HSF family protein
Niben101Scf04096g06005.1HSF family protein
Niben101Scf04122g06001.1HSF family protein
Niben101Scf04132g01004.1HSF family protein
Niben101Scf04313g01012.1HSF family protein
Niben101Scf04995g04005.1HSF family protein
Niben101Scf05056g00004.1HSF family protein
Niben101Scf05201g05003.1HSF family protein
Niben101Scf05787g01001.1HSF family protein
Niben101Scf05948g03017.1HSF family protein
Niben101Scf05948g04001.1HSF family protein
Niben101Scf06769g01010.1HSF family protein
Niben101Scf06814g01022.1HSF family protein
Niben101Scf07203g02006.1HSF family protein
Niben101Scf07391g04028.1HSF family protein
Niben101Scf07509g01001.1HSF family protein
Niben101Scf07663g01004.1HSF family protein
Niben101Scf08149g00015.1HSF family protein
Niben101Scf08341g10005.1HSF family protein
Niben101Scf08470g05002.1HSF family protein
Niben101Scf08995g00012.1HSF family protein
Niben101Scf10940g06011.1HSF family protein
Niben101Scf11512g01005.1HSF family protein
Niben101Scf11513g00004.1HSF family protein
Niben101Scf12112g02002.1HSF family protein
Niben101Scf13036g00009.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645